Publications

2020-today

2024

Kolesinski P, McGowan M, Botteaux A, Smeesters PR, Ghosh P. Conservation of C4BP-binding sequence patterns in Streptococcus pyogenes M and Enn proteins. J Biol Chem. 2024;300(7):107478. PubMed PMID: 38879009.

2023

Handa S*, Biswas T*, Chakraborty J, Paul BG, Ghosh P. Structural Requirements for Reverse Transcription by a Diversity-generating Retroelement. bioRxiv. 2023:2023.10.23.563531. *Contributed equally.

Maiti A, Buffalo CZ, Saurabh S, Montecinos-Franjola F, Hachey JS, Conlon WJ, Tran GN, Hassan B, Walters KJ, Drobizhev M, Moerner WE, Ghosh P, Matsuo H, Tsien RY, Lin JY, Rodriguez EA. Structural and photophysical characterization of the small ultra-red fluorescent protein. Nat Commun. 2023 Jul 12;14(1):4155. doi: 10.1038/s41467-023-39776-9. PMID: 37438348.

Wang KC, Kuliyev E, Nizet V, Ghosh P. A conserved three-dimensional pattern in a Streptococcus pyogenes M protein immunogen elicits M type cross-reactivity. J Biol Chem. 2023 Jun 28:104980. doi: 10.1016/j.jbc.2023.104980. Epub ahead of print. PubMed 37390991

Santos JC, Handa S, Fernandes LGV, Bleicher L, Gandin CA, de Oliveira-Neto M, Ghosh P, Nascimento A. Structural and biochemical characterization of Leptospira interrogans Lsa45 reveals a penicillin-binding protein with esterase activity. Process Biochem. 2023;125:141-53. PMC9836055. PubMed 36643388

2022

Spiegelman L, Bahn-Suh A, Montaño ET, Zhang L, Hura GL, Patras KA, Kumar A, Tezcan A, Nizet V, Tsutakawa S, Ghosh P. Strengthening of enterococcal biofilms by Esp. PLoS Pathog. 2022;18(9):e1010829. PMC9512215. PubMed 36103556

Kolesinski P, Wang KC, Hirose Y, Nizet VN, Ghosh P. An M protein coiled coil unfurls and exposes its hydrophobic core to capture LL-37. eLife  11:e77989. 2022 Jun 21;11:e77989. doi: 10.7554/eLife.77989. PMID: 35726694; PMCID: PMC9212996.

Hirose Y, Kolesinski P, Hiraoka M, Uchiyama S, Zurich RH, Kumaraswamy M, Bjanes E, Ghosh P, Kawabata S, Nizet V. Contributions of Stretpococcus pyogenes M87 protein to innate immune resistance and virulence. Microbial Pathogenesis. 2022 Aug;169:105636. doi: 10.1016/j.micpath.2022.105636. Epub 2022 Jun 17. PMID: 35724830.

2021

Mills JO, Ghosh P. Nonimmune antibody interactions of Group A Streptococcus M and M-like proteins. PLoS Pathogens 2021;17(2):e1009248. PubMed 33630944

Handa S, Reyna A, Wiryaman T, Ghosh P. Determinants of Adenine-mutagenesis in Diversity-Generating Retroelements. NAR 2021;49(2):1033-45. PubMed 33367793

2010-2019

2019

Handa S, Shaw KL, Ghosh P. Crystal structure of a Thermus aquaticus diversity-generating retroelement variable protein. PLoS One 2019;14(1):e0205618. PubMed 30629599

2018

Handa S, Jiang Y, Tao S, Foreman R, Schinazi RF, Miller JF, Ghosh P. Template-assisted synthesis of adenine-mutagenized cDNA by a retroelement protein complex. Nucleic Acids Res 2018;46(18):9711-25. PubMed 30007279

Wu L, Gingery M, Abebe M, Arambula D, Czornyj E, Handa S, Khan H, Liu M, Pohlschroder M, Shaw KL, Du A, Guo H, Ghosh P, Miller JF, Zimmerly S. Diversity-generating retroelements: natural variation, classification and evolution inferred from a large-scale genomic survey. Nucleic Acids Res 2018;46(1):11-24. PMC5758913. PubMed 29186518

2017

Ghosh P. Variation, Indispensability, and Masking in the M protein. Trends Microbiol. 2018;26(2):132-44. PMC5794653. PubMed 28867148

Valderrama JA, Riestra AM, Gao NJ, LaRock CN, Gupta N, Ali SR, Hoffman HM, Ghosh P, Nizet V. Group A streptococcal M protein activates the NLRP3 inflammasome. Nat Microbiol. 2017. PubMed 28784982

Paul BG, Burstein D, Castelle CJ, Handa S, Arambula D, Czornyj E, Thomas BC, Ghosh P, Miller JF, Banfield JF, Valentine D.L. Retroelement-guided protein diversification abounds in vast lineages of Bacteria and Archaea.Nat Microbiol. 2017;2:17045. PubMed 28368387

Dohrmann S, LaRock CN, Anderson EL, Cole JN, Ryali B, Stewart C, Nonejuie P, Pogliano J, Corriden R, Ghosh P, Nizet V. Group A Streptococcal M1 Protein Provides Resistance against the Antimicrobial Activity of Histones.Sci Rep. 2017;7:43039. PubMed 28220899

2016

Buffalo CZ, Bahn-Suh AJ, Hirakis SP, Biswas T, Amaro RE, Nizet V, Ghosh P. Conserved patterns hidden within group A Streptococcus M protein hypervariability recognize human C4b-binding protein.Nat Microbiol. 2016;1:16155. PubMed 27595425

Handa S, Paul BG, Miller JF, Valentine DL, Ghosh P. Conservation of the C-type lectin fold for accommodating massive sequence variation in archaeal diversity-generating retroelements. BMC Struct Biol. 2016;16(1):13. PubMed 27578274

Stewart CM, Buffalo CZ, Valderrama JA, Henningham A, Cole JN, Nizet V, Ghosh P. Coiled-coil destabilizing residues in the group A Streptococcus M1 protein are required for functional interaction. Proc Natl Acad Sci U S A. 2016 Aug 10. pii: 201606160. PubMed 27512043

2015

LaRock CN, Dohrmann S, Todd J, Corriden R, Olson J, Johannssen T, Lepenies B, Gallo RL, Ghosh P, Nizet V. Group A Streptococcal M1 Protein Sequesters Cathelicidin to Evade Innate Immune Killing. Cell Host Microbe 2015;18(4):471-7. PubMed 26468750

Paul BG, Bagby SC, Czornyj E, Arambula D, Handa S, Sczyrba A, Ghosh P, Miller JF, Valentine DL. Targeted diversity generation by intraterrestrial archaea and archaeal viruses. Nat Commun. 2015;6:6585. PubMed 25798780

2014

Hamaoka B, Ghosh P. Structure of the Essential Plasmodium Host Cell Traversal Protein SPECT1. PLoS One 2014;9(12):e114685.

Henningham A, Yamaguchi M, Aziz RK, Kuipers K, Buffalo CZ, Dahesh S, Choudhury B, Van Vleet J, Yamaguchi Y, Seymour LM, Ben Zakour NL, He L, Smith HV, Grimwood K, Beatson SA, Ghosh P, Walker MJ, Nizet V, Cole JN. Mutual Exclusivity of Hyaluronan and Hyaluronidase in Invasive Group A Streptococcus. J Biol Chem 2014;289(46):32303-15.

Anderson EL, Cole JN, Olson J, Ryba B, Ghosh P, Nizet V. The Fibrinogen-binding M1 Protein Reduces Pharyngeal Cell Adherence and Colonization Phenotypes of M1T1 Group A Streptococcus. J Biol Chem 2014;289(6):3539-46.

2013

Mukerjea R, Ghosh, P. Functionally essential interaction between Yersinia YscO and the T3S4 domain of YscP. J Bacti 2013;195(20):4631-8.

Arambula D, Wong W, Medhekar BA, Guo H, Gingery M, Czornyj E, Liu M, Dey S, Ghosh P, Miller JF. Surface display of a massively variable lipoprotein by a Legionella diversity-generating retroelement. Proc Natl Acad Sci U S A 2013;110(20):8212-7.

Uchiyama S, Andreoni F, Zurcher C, Schilcher K, Ender M, Madon J, Matt U, Ghosh P, Nizet V, Schuepbach RA, Zinkernagel AS. Coiled-coil irregularities of the M1 protein structure promote M1-fibrinogen interaction and influence group A Streptococcus host cell interactions and virulence. J Mol Med (Berl) 2013;91(7):861-9.

Alayyoubi M, Guo H, Dey S, Golnazarian T, Brooks GA, Rong A, Miller JF, Ghosh P. Structure of the essential diversity-generating retroelement protein bAvd and its functionally important interactions with reverse transcriptase. Structure 2013;21(2):266-76.

2012

Hui F, Scheib U, Hu Y, Sommer RJ, Aroian RV, Ghosh P. The structure and glycolipid-binding properties of the nematicidal protein Cry5B. Biochemistry 2012;51(49):9911-21.

Gamez A, Mukerjea R, Alayyoubi M, Ghassemian M, Ghosh P. The structure and interactions of the cytoplasmic domain of the Yersinia type III secretion protein YscD. J Bacteriol 2012;194(21):5949-58.

Bzymek KP, Hamaoka BY, Ghosh P. Two translation products of Yersinia yscQ assemble to form a complex essential to type III secretion. Biochemistry 2012;51(8):1669-77.

2011

Macheboeuf P, Ghosh P. Bases structurales du syndrome du choc toxique streptococcique. Med Sci (Paris) 2011;27(10):814-816.

Le Coq J, Ghosh P. Conservation of the C-type lectin fold for massive sequence variation in a Treponema diversity-generating retroelement. Proc Natl Acad Sci USA 2011;108(35):14649-53.

Macheboeuf P, Buffalo C, Fu C, Zinkernagel AS, Cole JN, Johnson JE, Nizet V, Ghosh P. Streptococcal M1 protein constructs a pathological host fibrinogen network. Nature 2011;472:64-68.

Ghosh P. The nonideal coiled coil of M protein and its multifarious functions in pathogenesis. Adv Exp Med Biol 2011;715:197-211.

Kao CY, Los FC, Huffman DL, Wachi S, Kloft N, Husmann M, Karabrahimi V, Schwartz JL, Bellier A, Ha C, Sagong Y, Fan H, Ghosh P, Hsieh M, Hsu CS, Chen L, Aroian RV. Global functional analyses of cellular responses to pore-forming toxins. PLoS Pathog 2011;7(3):e1001314.

Hanes MS, Reynolds KA, McNamara C, Ghosh P, Bonomo RA, Kirsch JF, Handel TM. Specificity and cooperativity at beta-lactamase position 104 in TEM-1/BLIP and SHV-1/BLIP interactions. Proteins 2011;79(4):1267-76.

2010

Rodgers L, Mukerjea R, Birtalan S, Friedberg D, Ghosh P. A solvent-exposed patch in chaperone-bound YopE is required for translocation by the type III secretion system. J Bacteriol 2010;192(12):3114-22.

Pentecost M, Kumaran J, Ghosh P, Amieva MR. Listeria monocytogenes internalin B activates junctional endocytosis to accelerate intestinal invasion. PLoS Pathog 2010;6(5):e1000900.

2000-2009

2009

Singh RK, Lau D, Noviello CM, Ghosh P, Guatelli JC. An MHC-I cytoplasmic domain/HIV-1 Nef fusion protein binds directly to the micro subunit of the AP-1 endosomal coat complex. PLoS One 2009;4(12):e8364.

Lauth X, von Kockritz-Blickwede M, McNamara CW, Myskowski S, Zinkernagel AS, Beall B, Ghosh P, Gallo RL, Nizet V. M1 protein allows Group A streptococcal survival in phagocyte extracellular traps through cathelicidin inhibition. J Innate Immun 2009;1(3):202-14.

2008

McNamara C, Zinkernagel AS, Macheboeuf P, Cunningham MW, Nizet V, Ghosh P. Coiled-coil irregularities and instabilities in group A Streptococcus M1 are required for virulence. Science 2008;319(5868):1405-8.

Miller JL, Le Coq J, Hodes A, Barbalat R, Miller JF, Ghosh P. Selective ligand recognition by a diversity-generating retroelement variable protein. PLoS Biol 2008;6(6):e131.

Rodgers L, Gamez A, Riek R, Ghosh P. The type III secretion chaperone SycE promotes a localized disorder-to-order transition in the natively unfolded effector YopE. J Biol Chem 2008;283(30):20857-63.

2007

Hrtska SC, Kemp MM, Munoz EM, Azizad O, Banerjee M, Raposo C, Kumaran J, Ghosh P, Linhardt RJ. Investigation of the mechanism of binding between internalin B and heparin using surface plasmon resonance. Biochemistry 2007;46(10):2697-706.

2006

Alto NM, Shao F, Lazar CS, Brost RL, Chua G, Mattoo S, McMahon SA, Ghosh P, Hughes TR, Boone C, Dixon JE. Identification of a bacterial type III effector family with G protein mimicry functions. Cell 2006;124(1):133-45.

Begley MJ, Taylor GS, Brock MA, Ghosh P, Woods VL, Dixon JE. Molecular basis for substrate recognition by MTMR2, a myotubularin family phosphoinositide phosphatase. Proc Natl Acad Sci U S A 2006;103(4):927-32. PMC1347996.

Mikolosko J, Bobyk K, Zgurskaya HI, Ghosh P. Conformational flexibility in the multidrug efflux system protein AcrA. Structure 2006;14(3):577-87.

2005

McMahon SA, Miller JL, Lawton JA, Kerkow DE, Hodes A, Marti-Renom MA, Doulatov S, Narayanan E, Sali A, Miller JF, Ghosh P. The C-type lectin fold as an evolutionary solution for massive sequence variation. Nat Struct Mol Biol 2005;12(10):886-92.

2004

Banerjee M, Copp J, Vuga D, Marino M, Chapman T, van der Geer P, Ghosh P. GW domains of the Listeria monocytogenes invasion protein InlB are required for potentiation of Met activation. Mol Microbiol 2004;52(1):257-71.

Marino M, Banerjee M, Copp J, Dramsi S, Chapman T, van der Geer P, Cossart P, Ghosh P. Characterization of the calcium-binding sites of Listeria monocytogenes InlB. Biochem Biophys Res Commun 2004;316(2):379-86.

Tanabe T, Chamaillard M, Ogura Y, Zhu L, Qiu S, Masumoto J, Ghosh P, Moran A, Predergast MM, Tromp G, Williams CJ, Inohara N, Nunez G. Regulatory regions and critical residues of NOD2 involved in muramyl dipeptide recognition. EMBO J 2004;23(7):1587-97.

Chatterji A, Ochoa W, Shamieh L, Salakian SP, Wong SM, Clinton G, Ghosh P, Lin T, Johnson JE. Chemical conjugation of heterologous proteins on the surface of Cowpea mosaic virus. Bioconjug Chem 2004;15(4):807-13.

Ghosh P. Process of protein transport by the type III secretion system. Microbiol Mol Biol Rev 2004;68(4):771-95.

2003

Copp J, Marino M, Banerjee M, Ghosh P, van der Geer P. Multiple regions of internalin B contribute to its ability to turn on the Ras-mitogen-activated protein kinase pathway. J Biol Chem 2003;278(10):7783-9.

Buetow L, Ghosh P. Structural elements required for deamidation of RhoA by cytotoxic necrotizing factor 1. Biochemistry 2003;42(44):12784-91.

Phillips RM, Six DA, Dennis EA, Ghosh P. In vivo phospholipase activity of the Pseudomonas aeruginosa cytotoxin ExoU and protection of mammalian cells with phospholipase A2 inhibitors. J Biol Chem 2003;278(42):41326-32.

Lawton JA, Ghosh P. Novel therapeutic strategies based on toll-like receptor signaling. Curr Opin Chem Biol 2003;7(4):446-51.

2002

Marino M, Banerjee M, Jonquieres R, Cossart P, Ghosh P. GW domains of the Listeria monocytogenes invasion protein InlB are SH3-like and mediate binding to host ligands. EMBO J 2002;21(21):5623-34.

Buetow L, Flatau G, Chiu K, Boquet P, Ghosh P. Strategies for the structural determination of the catalytic domain of Escherichia coli cytotoxic necrotizing factor 1. Acta Crystallogr D Biol Crystallogr 2002;58(Pt 2):366-9.

Birtalan SC, Phillips RM, Ghosh P. Three-dimensional secretion signals in chaperone-effector complexes of bacterial pathogens. Mol Cell 2002;9(5):971-80.

Que X, Ngo H, Lawton J, Gray M, Liu Q, Engel J, Brinen L, Ghosh P, Joiner KA, Reed SL. The cathepsin B of Toxoplasma gondii, toxopain-1, is critical for parasite invasion and rhoptry protein processing. J Biol Chem 2002;277(28):25791-7.

Ghosh P, Chapman T. Target specificity in leucine-rich repeat (LRR) proteins. In: Leong SA, Allen CA, Triplett EW, editors. Biology of Plant-Microbe Interactions. St. Paul, Minnesota: APS Press; 2002. p. 41-5.

2001

Buetow L, Flatau G, Chiu K, Boquet P, Ghosh P. Structure of the Rho-activating domain of Escherichia coli cytotoxic necrotizing factor 1. Nat Struct Biol 2001;8(7):584-8.

Birtalan S, Ghosh P. Structure of the Yersinia type III secretory system chaperone SycE. Nat Struct Biol 2001;8(11):974-8.

2000

Khan AR, Baker BM, Ghosh P, Biddison WE, Wiley DC. The structure and stability of an HLA-A*0201/octameric tax peptide complex with an empty conserved peptide-N-terminal binding site. J Immunol 2000;164(12):6398-405.

Marino M, Braun L, Cossart P, Ghosh P. A framework for interpreting the leucine-rich repeats of the Listeria internalins. Proc Natl Acad Sci U S A 2000;97(16):8784-8.

1990-1999

1999

Marino M, Braun L, Cossart P, Ghosh P. Structure of the lnlB leucine-rich repeats, a domain that triggers host cell invasion by the bacterial pathogen L. monocytogenes. Mol Cell 1999;4(6):1063-72.

1997

Wiener M, Freymann D, Ghosh P, Stroud RM. Crystal structure of colicin Ia. Nature 1997;385(6615):461-4.

Garboczi DN, Ghosh P, Utz U, Biddison WE, Wiley DC, Production, crystallization, and preliminary X-ray analysis of a human αβ T cell receptor, a peptide, and the class I MHC molecule HLA-A2. HLA – Genetic Diversity of HLA Functional and Medical Implication, Proceedings of the Twelfth International Histocompatibility Workshop and Conference. 1997.

1996

Garboczi DN, Utz U, Ghosh P, Seth A, Kim J, VanTienhoven EA, Biddison WE, Wiley DC. Assembly, specific binding, and crystallization of a human TCR-αβ with an antigenic Tax peptide from human T lymphotropic virus type 1 and the class I MHC molecule HLA-A2. J Immunol. 1996;157(12):5403-10.

Garboczi DN, Ghosh P, Utz U, Fan QR, Biddison WE, Wiley DC. Structure of the complex between human T-cell receptor, viral peptide and HLA-A2. Nature 1996;384(6605):134-41.

1995

Ghosh P, Amaya M, Mellins E, Wiley DC. The structure of an intermediate in class II MHC maturation: CLIP bound to HLA-DR3. Nature 1995;378(6556):457-62.

1994

Ghosh P, Mel SF, Stroud RM. The domain structure of the ion channel-forming protein colicin Ia. Nat Struct Biol 1994;1(9):597-604.

1993

Ghosh P, Mel SF, Stroud RM. A carboxy-terminal fragment of colicin Ia forms ion channels. J Membr Biol 1993;134(2):85-92.

1991

Ghosh P, Stroud RM. Ion channels formed by a highly charged peptide. Biochemistry 1991;30(14):3551-7.

1985-1989

1989

Stroud RM, McCarthy MP, Earnest JP, Shuster M, Ghosh P, Mitra AK.Molecular biology of the acetylcholine receptor. In: Sellin LC, Libelius R, Thesleff S, editors. Neuromuscular Junction. Amsterdam: Elsevier Science Publishers; 1989. p. 209-16.

1986

Leontis NB, Ghosh P, Moore PB. Effect of magnesium ion on the structure of the 5S RNA from Escherichia coli. An imino proton magnetic resonance study of the helix I, IV, and V regions of the molecule. Biochemistry 1986;25(23):7386-92.

1985

Leontis NB, Ghosh P, Moore PB. On the Conformational Properties of 5S RNA. In: Sarma RH, Sarma MH, editors. The Proceedings of the Fourth Conversation held at The University-SUNY, Albany NY, June 4-8, 1985: Biomolecular Stereodynamics. Schenectady, NY: Adenine Press. 1985. p. 287-306.